******************************************************************************** MEME - Motif discovery tool ******************************************************************************** MEME version 3.5.4 (Release date: ) For further information on how to interpret these results or to get a copy of the MEME software please access http://meme.nbcr.net. This file may be used as input to the MAST algorithm for searching sequence databases for matches to groups of motifs. MAST is available for interactive use and downloading at http://meme.nbcr.net. ******************************************************************************** ******************************************************************************** REFERENCE ******************************************************************************** If you use this program in your research, please cite: Timothy L. Bailey and Charles Elkan, "Fitting a mixture model by expectation maximization to discover motifs in biopolymers", Proceedings of the Second International Conference on Intelligent Systems for Molecular Biology, pp. 28-36, AAAI Press, Menlo Park, California, 1994. ******************************************************************************** ******************************************************************************** TRAINING SET ******************************************************************************** DATAFILE= ../data/oreganno_data/processed_data/regulons_for_one_factor/His2B_factor_binding_sites_sequences.fa ALPHABET= ACGT Sequence name Weight Length Sequence name Weight Length ------------- ------ ------ ------------- ------ ------ His2B_chr3L_20631214_206 1.0000 8 His2B_chr3L_20631233_206 1.0000 8 His2B_chr3L_20631225_206 1.0000 6 His2B_chr3L_20631244_206 1.0000 7 His2B_chr3L_20631203_206 1.0000 7 ******************************************************************************** ******************************************************************************** COMMAND LINE SUMMARY ******************************************************************************** This information can also be useful in the event you wish to report a problem with the MEME software. command: meme ../data/oreganno_data/processed_data/regulons_for_one_factor/His2B_factor_binding_sites_sequences.fa -dna -mod zoops -nmotifs 1 -revcomp -minw 6 -maxw 25 -dir /Users/jturatsi model: mod= zoops nmotifs= 1 evt= inf object function= E-value of product of p-values width: minw= 6 maxw= 8 minic= 0.00 width: wg= 11 ws= 1 endgaps= yes nsites: minsites= 2 maxsites= 5 wnsites= 0.8 theta: prob= 1 spmap= uni spfuzz= 0.5 em: prior= dirichlet b= 0.01 maxiter= 50 distance= 1e-05 data: n= 36 N= 5 strands: + - sample: seed= 0 seqfrac= 1 Letter frequencies in dataset: A 0.292 C 0.208 G 0.208 T 0.292 Background letter frequencies (from dataset with add-one prior applied): A 0.287 C 0.212 G 0.212 T 0.287 ******************************************************************************** ******************************************************************************** MOTIF 1 width = 6 sites = 5 llr = 21 E-value = 3.6e+000 ******************************************************************************** -------------------------------------------------------------------------------- Motif 1 Description -------------------------------------------------------------------------------- Simplified A 822:48 pos.-specific C 2:82:: probability G :8:222 matrix T :::64: bits 2.2 2.0 1.8 1.6 Information 1.3 ** content 1.1 *** * (6.1 bits) 0.9 *** * 0.7 **** * 0.4 ****** 0.2 ****** 0.0 ------ Multilevel AGCTAA consensus CAACTG sequence GG -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 sites sorted by position p-value -------------------------------------------------------------------------------- Sequence name Strand Start P-value Site ------------- ------ ----- --------- ------ His2B_chr3L_20631203_206 + 2 6.17e-04 C AGCTTA His2B_chr3L_20631214_206 + 1 8.45e-04 . AGCTGA TC His2B_chr3L_20631244_206 - 1 6.36e-03 A AGCCAG His2B_chr3L_20631233_206 + 2 1.03e-02 T AACGAA A His2B_chr3L_20631225_206 + 1 1.29e-02 . CGATTA -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 block diagrams -------------------------------------------------------------------------------- SEQUENCE NAME POSITION P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- His2B_chr3L_20631203_206 0.00062 1_[+1] His2B_chr3L_20631214_206 0.00085 [+1]_2 His2B_chr3L_20631244_206 0.0064 [-1]_1 His2B_chr3L_20631233_206 0.01 1_[+1]_1 His2B_chr3L_20631225_206 0.013 [+1] -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 in BLOCKS format -------------------------------------------------------------------------------- BL MOTIF 1 width=6 seqs=5 His2B_chr3L_20631203_206 ( 2) AGCTTA 1 His2B_chr3L_20631214_206 ( 1) AGCTGA 1 His2B_chr3L_20631244_206 ( 1) AGCCAG 1 His2B_chr3L_20631233_206 ( 2) AACGAA 1 His2B_chr3L_20631225_206 ( 1) CGATTA 1 // -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 position-specific scoring matrix -------------------------------------------------------------------------------- log-odds matrix: alength= 4 w= 6 n= 11 bayes= 1.69188 E= 3.6e+000 147 -9 -897 -897 -52 -897 191 -897 -52 191 -897 -897 -897 -9 -9 106 48 -897 -9 48 147 -897 -9 -897 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 position-specific probability matrix -------------------------------------------------------------------------------- letter-probability matrix: alength= 4 w= 6 nsites= 5 E= 3.6e+000 0.800000 0.200000 0.000000 0.000000 0.200000 0.000000 0.800000 0.000000 0.200000 0.800000 0.000000 0.000000 0.000000 0.200000 0.200000 0.600000 0.400000 0.000000 0.200000 0.400000 0.800000 0.000000 0.200000 0.000000 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 regular expression -------------------------------------------------------------------------------- [AC][GA][CA][TCG][ATG][AG] -------------------------------------------------------------------------------- Time 0.02 secs. ******************************************************************************** ******************************************************************************** SUMMARY OF MOTIFS ******************************************************************************** -------------------------------------------------------------------------------- Combined block diagrams: non-overlapping sites with p-value < 0.0001 -------------------------------------------------------------------------------- SEQUENCE NAME COMBINED P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- His2B_chr3L_20631214_206 5.06e-03 8 His2B_chr3L_20631233_206 6.01e-02 8 His2B_chr3L_20631225_206 2.56e-02 6 His2B_chr3L_20631244_206 2.52e-02 7 His2B_chr3L_20631203_206 2.47e-03 7 -------------------------------------------------------------------------------- ******************************************************************************** ******************************************************************************** Stopped because nmotifs = 1 reached. ******************************************************************************** CPU: jturatsi.scmbb.ulb.ac.be ********************************************************************************